Dr Bayés seminar, Molecular physiology of the synapse, that will be held Wednesday, September 16ht at…
Date / Time: Friday, March 5th 2021. 10:00h
Speaker: GERARD MAZÓN, Research group leader at the CNRS-Institute Gustave Roussy, Université Paris-Sud.
Title: “Sumo-directed control of the resolvase Yen1 in mitotic cells”
Members of the IBMB can find the link in your mailbox.
The modification of DNA damage response proteins with SUMO is an important mechanism to orchestrate a timely and orderly recruitment of repair factors to damaged sites. After replication stress and double-strand break formation a number of repair factors are sumoylated and interact with other sumoylated factors, including the nuclease Yen1. Yen1 plays a critical role to ensure genome instability and unperturbed chromosome segregation by removing covalently linked DNA intermediates that are formed by Homologous Recombination. In this seminar, I will show you recent data of my laboratory that demonstrates how Yen1 active pools are controlled by the sumo-targeted ubiquitinase Slx5-Slx8 to curb down its deleterious effects and how the important role of Yen1 is dependent on interactions mediated by non-covalent binding to sumoylated partners. Mutations in the motifs that allow sumo-mediated recruitment of Yen1 impair its ability to resolve DNA intermediates and result in increased genome instability and chromosome mis-segregation.