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Presentation

The Automated Crystallography Platform (PAC) is a shared service from IBMB-CSIC.

It offers researchers a complete set of methods to resolve the crystallographic structure of protein and nucleic acids.

Services offered:

  • Protein crystallization in standard SBS plates. Standard screenings and customized optimizations:
    More than 3000 standard starting conditions in more than 30 different screening plates.
  • Remote inspection of crystal growth.
  • X-ray diffraction in synchrotron.
  • DLS for general use.
  • SEC-MALS analysis of biological macromolecules [contact Pepita Pla: ppvcri@ibmb.csic.es]
  • Design and development of crystallography research projects.
  • Scientific and technical consulting.
  • Use of equipment for academia and companies.

Equipment

Main equipment:
  1. Tecan Evo 100 and 150 for screening and optimization construction.
  2. Art Robbins Phoenix protein dispenser.
  3. Douglas Instruments Oryx8 protein dispenser and simple optimization.
  4. Bruker Crystal Farms at 20ºC and 4ºC with remote access for crystal growth inspection.
  5. SEC-MALS Shimadzu-Wyatt
  6. DLS Malvern ZetaSizer
  7. Storage and fishing rooms at 20ºC and 4ºC

Prices

CONCEPT INTERNAL ACADEMIC OTHER
Std. Screenings (PACs, per plate) 19.58 36.88 40.39
Additives (price added to Std or optimization) 7.99 21 23.36
Optimization plate 13.48 28.96 31.72
Aliquot (1ml or 1µl if it is additive) 0.51 0.66 0.72
Optimization/customization in DW (0.2ml * 96 wells) 19.67 57.88 63.39
Optimization/customization in DW (0.5ml * 96 wells) 32.09 73.98 81.03
Optimization/customization in DW (1ml * 96 wells) 52.83 100.87 110.48
Optimization/customization in DW (1.8ml * 96 wells) 85.97 143.85 157.55
Deep Well (DW) 6.72 8.72 9.55
cover DW 5.55 7.19 7.88
plate MRC-2w 5.6 7.27 7.96
plate MRC-3w 6.78 8.79 9.63
plate cover MRC 1.49 1.94 2.13
Data processing, Structure solution, refinement, etc. (hourly rate) 0 51.39 56.28
pins cryo 8.54 11.07 12.12
SEC-MALS experiment 55.88 83.69 91.66
SEC-MALS additional sample 25.4 36.68 40.17
DLS 0.0 0.0 0.0

Notes:

  • Prices in €
  • Internal: founding groups
  • Academic: public research groups
  • Other: Companies
  • DLS free of charge for self service use
  • It is mandatory to request a budget for new projects
  • VAT not included

User corner

Access for registered users (calendar, farms, screenings, manuals, etc): https://pac.ibmb.csic.es:8084/

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