Presentation
The Automated Crystallography Platform (PAC) is a shared service from IBMB-CSIC.
It offers researchers a complete set of methods to resolve the crystallographic structure of protein and nucleic acids.
Services offered:
- Protein crystallization in standard SBS plates. Standard screenings and customized optimizations:
More than 3000 standard starting conditions in more than 30 different screening plates.
- Remote inspection of crystal growth.
- X-ray diffraction in synchrotron.
- DLS for general use.
- SEC-MALS analysis of biological macromolecules [contact Mònica Buxaderas: mobcri@ibmb.csic.es]
- Design and development of crystallography research projects.
- Scientific and technical consulting.
- Use of equipment for academia and companies.
Equipment
Main equipment:
- Tecan Evo 100 and 150 for screening and optimization construction.
- Art Robbins Phoenix protein dispenser.
- Douglas Instruments Oryx8 protein dispenser and simple optimization.
- Bruker Crystal Farms at 20ºC and 4ºC with remote access for crystal growth inspection.
- SEC-MALS Shimadzu-Wyatt
- DLS Malvern ZetaSizer
- Storage and fishing rooms at 20ºC and 4ºC
Prices
| CONCEPT | INTERNAL | ACADEMIC | OTHER |
|---|---|---|---|
| Std. Screenings (PACs, per plate) | 19.58 | 36.88 | 40.39 |
| Additives (price added to Std or optimization) | 7.99 | 21 | 23.36 |
| Optimization plate | 13.48 | 28.96 | 31.72 |
| Aliquot (1ml or 1µl if it is additive) | 0.51 | 0.66 | 0.72 |
| Optimization/customization in DW (0.2ml * 96 wells) | 19.67 | 57.88 | 63.39 |
| Optimization/customization in DW (0.5ml * 96 wells) | 32.09 | 73.98 | 81.03 |
| Optimization/customization in DW (1ml * 96 wells) | 52.83 | 100.87 | 110.48 |
| Optimization/customization in DW (1.8ml * 96 wells) | 85.97 | 143.85 | 157.55 |
| Deep Well (DW) | 6.72 | 8.72 | 9.55 |
| cover DW | 5.55 | 7.19 | 7.88 |
| plate MRC-2w | 5.6 | 7.27 | 7.96 |
| plate MRC-3w | 6.78 | 8.79 | 9.63 |
| plate cover MRC | 1.49 | 1.94 | 2.13 |
| Data processing, Structure solution, refinement, etc. (hourly rate) | 0 | 51.39 | 56.28 |
| pins cryo | 8.54 | 11.07 | 12.12 |
| SEC-MALS experiment | 55.88 | 83.69 | 91.66 |
| SEC-MALS additional sample | 25.4 | 36.68 | 40.17 |
| DLS | 0.0 | 0.0 | 0.0 |
Notes:
- Prices in €
- Internal: founding groups
- Academic: public research groups
- Other: Companies
- DLS free of charge for self service use
- It is mandatory to request a budget for new projects
- VAT not included
User corner
Access for registered users (calendar, farms, screenings, manuals, etc): https://pac.ibmb.csic.es:8084/
