New publication in eLife of the Jordan Lab in collaboration with the IBMB Imaging Platform showing that…
JmjC family of histone demethylases form nuclear condensates
With our work, we demonstrate that the JmjC-family of histone demethylases contain low complexity domains and intrinsically disordered regions (IDR), that they use to cluster by phase separation. The data suggest that clustering via phase separation is a common feature that JMJC-demethylases utilize to achieve the competent concentration of molecules in time and space within the cell nucleus.
Abstract
JmjC-family of lysine demethylases (JMJC-KDMs) plays an essential role in controlling gene expression and chromatin structure. In most cases, their function has been attributed to the demethylase activity. However, accumulating evidence demonstrates that these proteins play roles distinct from histone demethylation. This raises the possibility that they might share domains that contribute to their functional outcome. Here, we show that the JMJC-KDMs contain low complexity domains and intrinsically disordered regions (IDR), which in some cases reached 70% of the protein. Our data revealed that PHF2, KDM2A, and KDM4B cluster by phase separation in vitro and in vivo. Moreover, our molecular analysis demonstrated that the PHF2-IDR is essential for regulating transcription. These data suggest that clustering via phase separation is a common feature that JMJC-KDMs utilize to facilitate their functional responses. Our study uncovers a novel potential function for the JMJC-KDM family that sheds light on the mechanisms to achieve the competent concentration of molecules in time and space within the cell nucleus.
Reference:
Vicioso-Mantis M, Aguirre S, Martínez-Balbás MA. JmjC family of histone demethylases form nuclear condensates. International Journal of Molecular Sciences. 2022, 23(14):7664. https://www.mdpi.com/1422-0067/23/14/7664. doi: 10.3390/ijms23147664.