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Media Kitchen

The service provides liquid and solid culture media for E. coli (LB and LB plus different antibiotics),S. cerevisiae (YPD and SDC drop outs) and D. melanogaster. A complete set of the most used buffers in cell and molecular biology are also offered. The users can either utilize the media and buffers in stock or order it in advance if an important quantity is required. The IBMB media kitchen mostly serve groups working at the IBMB, but external services can also be provided exceptionally.

A full set of media and buffers prepared are listed below.

Equipment

Media

LB liquid
LB agar plates
LB agar plates ampicillin
LB agar plates kanamycin
LB agar plates chloramphenicol
YPD liquid
YPD agar plates
YPD agar plates geneticin
SDC* single drop outs liquid
SDC* single drop outs agar plates
SDC* double drop outs liquid
SDC* double drop outs agar plates
SDC* triple drop outs liquid
SDC* triple drop outs agar plates
SDC* cuadruple drop outs liquid
SDC* cuadruple drop outs agar plate
*SDC drop outs can be –Ura, -Lys, -Leu, -His, -Trp. -Ade

Buffers

NaCl 5M
Coomassie destaining
SDS 10 %
Tris 1M, different pH adjusted
Hepes 1M, different pH adjusted
EDTA 0.5M
TAE (Tris-Acetate-EDTA) x 50
TBS (Tris-Buffered-Saline) x 10
PBS (Phosphate-Buffered-Saline) x 10
TBE (Tris-Borate EDTA) x 10
SDS-PAGE Running Buffer x 10
SDS-PAGE Transfer buffer x 10

Prices

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