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Lab presentation

Genomic functions take place in chromatin, not in naked DNA. In recent years, our knowledge about the regulation of chromatin functions has improved thanks to the identification of components and mechanisms that modify its structural and functional properties, such as remodelling complexes, histone modifications (acetylation, methylation…) and the corresponding enzymes, histone variants that localise to specific chromosome locations, structural non-histone proteins that contribute to the functional properties of specific chromatin domains, among others.

Our research focuses on the study of the molecular basis of chromatin function, their regulation during cell cycle and development, and their alteration in human pathologies. More specifically, our work addresses:

(I) the analysis of the contribution of linker histones H1 to the epigenetic regulation of chromatin structure and function

(II) the study of the factor and mechanisms involved in formation and maintenance of the tridimensional (3D) organization of chromatin inside the nucleus

(III) the analysis of the epigenetic factors and mechanism that regulate transcription

(IV) the study of centromeric chromatin and its contribution to chromosome segregation and mitosis progression

Projects

I. Linker histones H1. We study the contribution of linker histones H1 to the epigenetic regulation of chromatin structure and function during development and differentiation, and their role in maintenance of genome stability

II. 3D genome structure. We study the factors and mechanisms involved in maintenance and formation of the tridimensional organization of chromatin inside the nucleus and their role in the regulation of genome functions.

III. Epigenetic regulation of transcription. We study the epigenetic factors and mechanisms that regulate transcription initiation, elongation and termination

IV. Centromere structure and function. We seek to determine the structural and functional properties of centromeric chromatin and its contribution to chromosome segregation and mitosis progression.

Selected publications

Selected publications in the last 10 years


Climent-Cantó, A. Carbonell, S. Tamirisa, L. Henn, I. M. Boros and F. Azorín (2021) “The tumour suppressor brain tumour (Brat) regulates linker histone dBigH1 expression in the Drosophila female germline and the early embryo” Open Biology, 11, 200408. doi: 10.1098/rsob.20.0408


Torras-Llort, S. Medina-Giró, P. Escudero-Ferruz, Z. Lipinszki, O. Moreno-Moreno, Z. Karman, M.R. Przewloka and F. Azorín (2020) “A fraction of barrier-to-autointegration factor (BAF) associates with centromeres and controls mitosis progression” Communications Biology, 3, 454. doi: 10.1038/s42003-020-01182-y


Climent-Cantó, A. Carbonell, M. Tatarski, O. Reina, P. Bujosa, J. Font-Mateu, J. Bernués, M. Beato and F. Azorin (2020) “The embryonic linker histone dBigH1 alters the functional state of active chromatin” Nucleic Acids Research, 48, 4147-4160. doi: 10.1093/nar/gkaa122


Moreno-Moreno, M. Torras-Llort and F. Azorín (2019) “The E3-ligases SCFPpa and APC/CCdh1 co-operate to regulate CENP-ACID expression across the cell cycle” Nucleic Acids Research, 47, 3395-3406. doi: 10.1093/nar/gkz060


Carbonell, S. Pérez-Montero, P. Climent, O. Reina and F. Azorín (2017) “The germilne linker histone dBigH1 and the translational regulator Bam form a repressor loop essential for male germ stem cell differentiation” Cell Reports, 21, 3178-3189. doi: 10.1016/j.celrep.2017.11.060


Bayona-Feliu, A. Casas-Lamesa, O. Reina, J. Bernués and F. Azorín (2017) “Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin”. Nature Communications, 18, 283. doi: 10.1038/s41467-017-00338-5


Kessler, J. Tisserand, J. Font-Burgada, O. Reina, L. Coch, C. Stephan-otto Attolini, I. Garcia-Bassets and F. Azorín (2015) “The ubiquitin receptor dDsk2 regulates H2Bub1 and RNA polymerase II pausing at dHP1c complex target genes” Nature Communications. 6:7049. doi: 10.1038/ncomms8049


Font-Burgada, O. Reina, D. Rossell and F. Azorín (2014) “chroGPS, a global chromatin positioning system for the functional analysis and visualization of the epigenome” Nucleic Acids Research, 42, 2126-2137. doi: 10.1093/nar/gkt1186


Pérez-Montero, A. Carbonell, T. Morán, A. Vaquero and F. Azorín (2013) “The embryonic linker histone H1 variant of Drosophila, dBigH1, regulates zygotic genome activation” Developmental Cell, 26, 578-590. doi: 10.1016/j.devcel.2013.08.011


Lloret-Llinares, S. Pérez-Lluch, D. Rossell, T. Morán, J. Ponsa-Cobas, H. Auer, M. Corominas and F. Azorín (2012) “dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes” Nucleic Acids Research, 40, 9493-9505. doi: 10.1093/nar/gks773


Bonet-Costa C, Vilaseca M, Diema C, Vujatovic O, Vaquero A, Omeñaca N, Castejón L, Bernués J, Giralt E, Azorín F. (2012) “Combined bottom-up and top-down mass spectrometry analyses of the pattern of post-translational modifications of Drosophila melanogaster linker histone H1” Journal of Proteomics, 75, 4124-4138. doi: 10.1016/j.jprot.2012.05.034


Vujatovic, K. Zaragoza, A. Vaquero, O. Reina, J. Bernués and F. Azorín (2012) “Drosophila melanogaster linker histone dH1 is required for transposon silencing and to preserve genome integrity” Nucleic Acids Research, 40, 5402-5414. doi: 10.1093/nar/gks224

All publications

More than 100 publications.

For a full list of publications go to: https://pubmed.ncbi.nlm.nih.gov/?term=azorin+f&sort=date&size=50

Project funding

Título: Regulación epigenética de la organización, estructura y función de la cromatina

Referencia: PGC2018-094538-B-I00 Entidad financiadora: MCINN.

Fecha de inicio: 2019. Fecha de finalización: 2021. Cuantía de la subvención: 336.017€

Tipo de participación: Investigador Principal

Proyecto PGC2018-094538-B-I00 financiado por:


Título: Nuevos factores y mecanismos epigenéticos implicados en la regulación de la estructura y función de la cromatina

Referencia: BFU2015-65082-P Entidad financiadora: MINECO, PN2012.

Fecha de inicio: 2016. Fecha de finalización: 2018. Cuantía de la subvención: 403.172€

Tipo de participación: Investigador Principal

Proyecto BFU2015-65082-P financiado por:


Título: Regulación epigenética de las funciones de la cromatina

Referencia: BFU2012-30724 Entidad financiadora: MINECO, PN2012.

Fecha de inicio: 2013. Fecha de finalización: 2015. Cuantía de la subvención: 468.000€

Tipo de participación: Investigador Principal


Título: Regulación epigenética de la estructura y función de la cromatina

Referencia: BFU2009-07111 Entidad financiadora: MCINN, PN2009.

Fecha de inicio: 2010. Fecha de finalización: 2012. Cuantía de la subvención: 716.632€

Tipo de participación: Investigador Principal


Título: Epigenética: mecanismos y enfermedad

Referencia: CSD2006-49 Entidad financiadora: MEC. Consolider Ingenio 2010.

Coordinador: Dr.M. Beato

Fecha de inicio: 2006. Fecha de finalización: 2012. Cuantía de la subvención: 509.999€

Tipo de participación: Investigador Principal

Vacancies/Jobs

PhD students and Postdoctoral fellows interested in joining our group are welcome to apply at anytime

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