Isabel Usón

Structural Biology Unit, Barcelona

ICREA Research Professor

Garib Murshudov

Laboratory of Molecular Biology, Cambridge

Programme

19 September

8:30      Participants registration

9:00      Welcome by Isabel Usón

9:10      Introduction by Garib Murshudov

Session I – Overview and principles

9:15      Richard Henderson (LMB-MRC, Cambridge, UK)

“An overview of cryoEM”

10:00    Sjors Scheres (LMB-MRC, Cambridge, UK)

“A Bayesian view on cryo-EM structure determination”

10:45    Coffee and poster presentation

11:15    José María Valpuesta (CNB-CSIC, Madrid)

“Combining biochemical and biophysical techniques and low-resolution EM maps for the generation of quasi-atomic models: the case of molecular chaperone complexes”

12:00    Holger Stark (MPIBPC, Göttingen, Germany)

“How to overcome biochemical limitations in sample preparation for high-resolution structure determination of macromolecular complexes by cryo-EM and X-ray crystallography”

12:45    Lunch

Session II – Solution and reconstruction

15:00    Airlie McCoy (CIMR, University of Cambridge, UK)

“Solving X-ray crystal structures with cryo-EM reconstructions”

15:45    Randy Read (CIMR, University of Cambridge, UK)

“Repurposing molecular replacement algorithms for docking models in Cryo-EM reconstructions”

16:30    Coffee and poster presentation

17:15    Paul Emsley (LMB-MRC, Cambridge, UK)

“Model-building in Low Resolution and Cryo-EM maps using Coot”

18:00    Tristan Croll (CIMR, University of Cambridge, UK)

“ISOLDE: interactive model building in a physically realistic environment”

18:45    End of the day

 

20 September

Session III – Model Building to automation

9:15      Jon Agirre (YSBL, University of York, UK)

“Automated model building in cryoEM maps”

10:00    José María Carazo (CNB-CSIC, Madrid)

“New approaches to the analysis of cryoEM maps quality”

10:45    Coffee and poster presentation

11:15    Elena Orlova (University of London, UK)

“Structural studies of heterogeneous assemblies”

12:00    Tom Terwilliger (Los Alamos National Laboratory, USA)

“Model-building using cryo-EM and crystallographic maps “

12:45    Lunch

Session IV – Refinement and Global Initiatives

15:00    Garib Murshudov (LMC-MRC, Cambridge, UK)

“Refinement of atomic models into cryo-EM maps using REFMAC5”

15:45    Pavel Afonine (Lawrence Berkeley National Laboratory, Berkeley CA, USA)

“New real space refinement methods for cryo-EM”

16:30    Coffee and poster presentation

17:15    Tom Burnley (CCPEM, UK)

“Introducing the Collaborative Computational Project for Electron cryo-Microscopy: CCP-EM”

18:00    Paula da Fonseca (LMB-MRC, UK)

“The emerging role of CryoEM in drug discovery and development”

18:45    End of the day

 

21 September

Session V – Application and Future prospects 

9:15      Werner Kühlbrandt (Max-Plank Institut für Biophysik)

“Investigating membrane protein structure and function by cryo-EM”

10:00    José Castón (CNB-CSIC, Madrid)

“Cryo-EM at near-atomic resolution of icosahedral viruses”

10:30    Coffee and poster presentation

11:15    Abhay Kotecha (Division of Structural biology, University of Oxford, UK)

“CryoEM illuminates the world of the Picornaviruses”

11:45    Rafael Fernandez Leiro (CNIO, Madrid)

“Mechanistic insights on DNA replication and repair by cryo-EM”

12:15    Paola Fucini (CIC-Biogune, Bilbao)

“Structural studies of functional ribosome complexes”

13:00    Concluding remarks

13:15    End