Spinning disk confocal microscope (PerkinElmer Ultraview ERS)
The PerkinElmer UltraVIEW system (PerkinElmer Life Sciences Inc., MA, USA) is a Yokogawa (Yokogawa Corp. Japan) Nipkow Spinning Disk Confocal System. It uses a spinning disk with multiple pinholes to achieve confocality (e.g. the rejection of out-of-focus light). Emission light from the sample passes through the pinholes to generate a confocal image of the sample that can be detected with an EMCCD (Electron Multiplification Charge-Coupled Device) camera. Spinning disk confocal systems have 2 main advantages over conventional Laser Scanning Confocal Microscopes (LSCM):
- Higher imaging speed: up to 360frames/sec compared to 0.5-1 frames per second in a LSC
- Lower photo-toxicity: around 5 times less than a LSCM, probably due to the fact that the system splits the laser light into thousands of minibeams.
It is specially suited for live 4D imaging of fast cellular processes and for FRAP (fluorescence recovery after photobleaching) and related experiments.