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Leica TCS-SP5 Confocal Microscope (Leica Microsystems, Wetzlar, Germany)


Laser scanning confocal systems have major imaging advantages:

  • Light rays from outside the focal plane will not be recorded, thus allowing for optical sectioning
  • The object can be scanned in x/y-direction as well as in z-direction (along the optical axis)
  • Upon processing, many slices can be superimposed, giving an extended focus image which can only be achieved in a conventional microscope by reducing the aperture and thus sacrificing resolution

Additionally, the SP5 TSC system provides a high efficiency spectral detection, which together with the AOBS (Acousto-Optical Beam Splitter) enables the simultaneous imaging of up to 4 different wavelengths at high resolution. The system covers a wide range of confocal applications, including a number of other advanced techniques such as FRAP, FRET, FLIP and Photoconversion, which are facilitated by user-friendly wizards designed to the purpose.


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